3、调控网络：实现了复杂调控网络的自动化设计，编辑细胞进行逻辑运算，成功构建了能显示7段数码管的工程菌。自动化设计并改进了基于CRISPR系统的调控网络，实现了肠道微生物的基因回路运算。
国家高层次人才计划青年项目
国家优秀自费职工奖
宾夕法尼亚州立大学优秀博士论文奖
代表性论文
1.
Shuyi Zhang and Donald A. Bryant. (2011). The tricarboxylic acid cycle in cyanobacteria.
Science, 334, 1551-1553, DOI: 10.1126/science.1210858
- This work was featured as cover story in Chapter 3 of the classic microbiology textbook Brock Biology of Microorganisms (14th Edition).
2. Kenchappa G. Kumaraswamy, Xiao Qian, Tiago Guerra,
Shuyi Zhang, Donald A. Bryant and Charles G. Dismukes. (2013). Reprogramming the glycolytic pathway for increased hydrogen production in cyanobacteria: metabolic engineering of NAD+-dependent GAPDH.
Energy & Environmental Science, 6(12):3722-3731, DOI: 10.1039/C3EE42206B.
3.
Shuyi Zhang, Gaozhong Shen, Zhongkui Li, John H. Golbeck and Donald A. Bryant. (2014). Vipp1 is essential for the biogenesis of Photosystem I but not thylakoid membranes in Synechococcus sp. PCC 7002.
Journal of Biological Chemistry, 289(23), 15904-15914, DOI: 10.1074/jbc.M114.555631.
4. Fei Gan,
Shuyi Zhang, Nathan C. Rockwell, Shelley S. Martin, Clark J. Lagarias and Donald A. Bryant. (2014) Extensive remodeling of a cyanobacterial photosynthetic apparatus in Far-Red light. Science, 345, 1312-1317, DOI: 10.1126/science.1256963
- This work was described as “text book changing stuff” by Professor Bill Rutherford from Imperial College London.
5. Hans C. Bernstein, Allan Konopka, Matthew R. Melnicki, Eric A. Hill, Leo A. Kucek,
Shuyi Zhang, Gaozhong Shen, Donald A. Bryant and Alexander S. Beliaev. (2014) Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of
Synechococcus sp. PCC 7002.
Frontiers in Microbiology, 5, 488, DOI: 10.3389/fmicb.2014.00488.
6.
Shuyi Zhang and Donald A. Bryant. (2014) Learning new tricks from an old cycle: the TCA cycles of cyanobacteria, algae and plants.
Perspectives in Phycology, 2, 73-86,
Invited review article, DOI: 10.1127/pip/2014/0016.
7.
Shuyi Zhang and Donald A. Bryant. (2015) Biochemical validation of the the glyoxylate cycle in the cyanobacterium
Chlorogloeopsis fritschii PCC 9212,
Journal of Biological Chemistry 290 (22), 14019-14030, DOI: 10.1074/jbc.M115.648170.
8.
Shuyi Zhang, Yang Liu and Donald A. Bryant. (2015) Metabolic engineering of
Synechococcus sp. PCC 7002 to produce poly-3-hydroxybutyrate and poly-3-hydroxybutyrate-co-4-hydroxybutyrate,
Metabolic Enginneering, 32, 174-183, DOI: 10.1016/j.ymben.2015.10.001.
9. Xiao Qian, Kenchappa G. Kumaraswamy,
Shuyi Zhang, Colin Gates, Donald A. Bryant and Charles G. Dismukes. (2015) Inactivation of nitrate reductase alters metabolic branching of carbohydrate fermentation in the cyanobacterium
Synechococcus sp. strain PCC 7002.
Biotechnology & Bioengineering, 113(5):979-988, DOI: 10.1002/bit.25862.
10. Anagha Krishnan,
Shuyi Zhang, Yang Liu, Kinan A Tadmori, Donald A Bryant and G Charles Dismukes (2015) Consequences of ccmR deletion on respiration, fermentation and H2 metabolism in cyanobacterium
Synechococcus sp. PCC 7002.
Biotechnology & Bioengineering, 113(7):1448-1459, DOI: 10.1002/bit.25913.
11.
Shuyi Zhang, Xiao Qian, Shannon Chang, G Charles Dismukes and Donald A Bryant (2016) Natural and synthetic variants of the tricarboxylic acid cycle in cyanobacteria: introduction of the GABA shunt into
Synechococcus sp. PCC 7002.
Frontiers in Microbiology, 7, 1972, DOI: 10.3389/fmicb.2016.01972.
12.
Shuyi Zhang and Christopher A. Voigt. (2018) Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design.
Nucleic Acids Research, gky884, DOI: 10.1093/nar/gky884.
13. Kenchappa G Kumaraswamy, Anagha Krishnan, Gennady Ananyev,
Shuyi Zhang, Donald A. Bryant and G Charles Dismukes. (2019) Crossing the Thauer limit: Rewiring Cyanobacterial Metabolism to Maximize Fermentative H
2 Production.
Energy & Environmental Science, 12, 1035-1045, DOI: 10.1039/C8EE03606C.
14. Jonghyeon Shin,
Shuyi Zhang, Bryan S. Der, Alec A.K. Nielsen, and Christopher A. Voigt. (2020) Programming Escherichia coli to function as a digital display.
Molecular Systems Biology, 16: e9401, DOI: 10.15252/msb.20199401. Highlighted as
Cover Article.
15. Mao Taketani, Jianbo Zhang,
Shuyi Zhang, Alexander J. Triassi, Yu-Ja Huang, Linda G. Griffith & Christopher A. Voigt. (2020) Genetic circuit design automation for the gut resident species
Bacteroides thetaiotaomicron.
Nature Biotechnology, DOI: 10.1038/s41587-020-0468-5.
16. Ye Chen,
Shuyi Zhang, Eric M. Young, and Christopher A. Voigt. (2020) Genetic circuit design automation for yeast.
Nature Microbiology, In minor revision.
